Hi how i can make a new tetramer protein i have made a monomer by alfa fold now i want to make homotetraner by docking
hi,
the procedure may depend on the information you have, but please start checking this tutorial HADDOCK 2.4 CASP-CAPRI T70 ab-initio docking tutorial – Bonvin Lab which explains how to perform ab-initio (i.e. with no prior information) docking of symmetrical homo-multimers. If you have some information on the interface consider adding it to the docking by means of ambiguous interaction restraints (check other tutorials such as HADDOCK2.4 basic protein-protein docking tutorial – Bonvin Lab)
In case you prefer to use HADDOCK3 (command line-only), have a look at this tutorial (for a symmetric homotrimer): haddock3/examples/docking-protein-homotrimer/docking-protein-homotrimer-test.cfg at main · haddocking/haddock3 · GitHub
Hope it helps.
Marco