Hello,
I tried to dock an inserted domain 2 to a domain 1. The domains are arranged as follows in the sequence:
111111111111222222222222221111111111111111111111
Using the easy interface, for protein 1, I specified the two adjacent residues (213, 214) as active and for the 2nd protein I specified the N- and C-termini as active (1 and 117). Unfortunately, the docking failed with the error message below (http://milou.science.uu.nl/serviceresults/HADDOCK2.2/5538826474/PositiveContrl) :
TOTAL NUMBER OF DISTANCE RESTRAINTS FOR RIGID BODY DOCKING IS ZERO!
CONTROL YOUR PARAMETER SETTINGS AND RESTRAINT DEFINITIONS
STRUCTURE NUMBER 601
I would be grateful for any help, or info if that approach is possible or not.
Many thanks
Andreas