Docking proteins with one set of ambigious constraints

HADDOCK
1

The HADDOCK category is meant to discuss any HADDOCK-related issue. For general information about HADDOCK refer to HADDOCK – Bonvin Lab

I am trying to dock two proteins, A and B.

I have NMR data supplying ambiguous constraints for protein A, but no ambiguous constraints for protein B.

I have MS data that gives me unambiguous constrains for both proteins A and B.

HADDOCK will not let me submit a calculation with only protein A having ambiguous constraints.

2

Define A as active and the entire surface of B as passive (the server has an auutomatic option for this)

And add your MS data as unambiguous restraints

Does required expert level access.

3

Worked like a charm!

Thank you so much for the speedy response and free program!!!