Distorted output structures


I’ve installed local version of haddock2.4 and I’m trying to dock large multi-chain complexes (>10 chains).

I followed the protein-protein docking tutorial and, on top of that, I only modified the run.cns file turning on the center of mass restraints and increasing the sampling as it is stated in this post:

Runs seems to complete successfully but in the ave.pdb results I find completely distorted structures. I also tried using just 2 chains, and the amount of distortion seems lower but still the structures are completely destroyed.

Do you have any idea of why this is happening?

The average file has no meaning…especially not if many different models are generated. Look at the individual models.

Oh that was quite simple.
I got tricked checking an avg.pdb file from a sample run you provided somewhere, which actually contains a good structure.

Sorry for the silly question and thanks for your answer :slight_smile:

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