Error in docking

when I’m using HADDOCK for docking it shows error-

There was an inconsistency in your data
Error message
First PDB file contains multiple residues with number 28 in chain A

ATOM 1 N SER A 28 64.897 12.514 42.098 1.00 0.00 N
ATOM 2 CA SER A 28 63.960 11.468 42.495 1.00 0.00 C
ATOM 3 C SER A 28 62.866 12.027 43.375 1.00 0.00 C
ATOM 4 O SER A 28 63.065 12.168 44.608 1.00 0.00 O
ATOM 5 CB SER A 28 64.701 10.295 43.183 1.00 0.00 C
ATOM 6 OG SER A 28 65.615 9.624 42.309 1.00 0.00 O
Thanks in advance.

The HADDOCK category is meant to discuss any HADDOCK-related issue. For general information about HADDOCK refer to

you might have a residue with multiple occupancy values. If this is the case, you can use our new webserver pdb-tools ( ) and use the script: pdb_selaltloc to select only one version of the residue and make your pdb ready for docking.

There must be another residue with number 28…

how can i resiolve this issue???

Check you PDB file for duplications of residue numbers!!!

my PDB file has 4 chains and I’m using only chain A and I checked my file there is no duplicity in chain A.

Then simply try to remove all chains before uploading to the server.

Did you tell the server to only use chain A?

yes I’ll tell the chain A