# Whisky error - cannot have the final result

**URL:** <https://ask.bioexcel.eu/t/whisky-error-cannot-have-the-final-result/4399>\
**Category:** Uncategorized\
**Created:** [July 4, 2023, 1:24pm UTC](https://ask.bioexcel.eu/t/whisky-error-cannot-have-the-final-result/4399 "2023-07-04T13:24:45Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![barbarainb](https://dub1.discourse-cdn.com/flex013/user_avatar/ask.bioexcel.eu/barbarainb/32/794_2.png) [@barbarainb](https://ask.bioexcel.eu/u/barbarainb)\
**Post date:** [July 4, 2023, 1:24pm UTC](https://ask.bioexcel.eu/t/whisky-error-cannot-have-the-final-result/4399/1 "2023-07-04T13:24:45Z")

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I managed to “run” whisky but i get the following error:

I have inputed a protein structure predction, with chain A to be analyzed and as protein alignment i used “8J1N” - ucp1 protein from [https://www.rcsb.org/structure/8J1N](https://www.rcsb.org/structure/8J1N), what is happening or what could ia change here ? thanks in advance

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command: /opt/software/whiscy/whiscy\_setup.py ENSMUSG00000031710\_hgfemale\_Ucp1\_seq\_model1.pdb A --hssp 8J1N  
exit status: 1  
log:  
whiscy\_setup [INFO] PDB structure with chain A saved to ENSMUSG00000031710\_hgfemale\_Ucp1\_seq\_model1\_A.pdb  
whiscy\_setup [INFO] Atom accessibility calculated to ENSMUSG00000031710\_hgfemale\_Ucp1\_seq\_model1\_A.rsa  
whiscy\_setup [INFO] Surface and buried residues calculated  
whiscy\_setup [INFO] Downloading HSSP alignment…  
whiscy\_setup [ERROR] HSSP file could not be generated  
Error is: \<urlopen error ftp error: URLError(“ftp error: error\_perm(‘550 Failed to change directory.’,)”,)\>

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<div class="post-metadata">

**Author:** ![amjjbonvin](https://dub1.discourse-cdn.com/flex013/user_avatar/ask.bioexcel.eu/amjjbonvin/32/23_2.png) [@amjjbonvin](https://ask.bioexcel.eu/u/amjjbonvin)\
**Post date:** [July 5, 2023, 9:03pm UTC](https://ask.bioexcel.eu/t/whisky-error-cannot-have-the-final-result/4399/2 "2023-07-05T21:03:30Z")

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Does this particular prediction fail?  
Did you try another system?

It seems related to the access to the hssp entry