# Using lipids in HADDOCK **URL:** \ **Category:** HADDOCK\ **Created:** [July 6, 2022, 3:12pm UTC](https://ask.bioexcel.eu/t/using-lipids-in-haddock/3794 "2022-07-06T15:12:47Z")\ **Posts on this page:** 3\ **Page:** 1
**Author:** ![Davidswm1](https://avatars.discourse-cdn.com/v4/letter/d/3da27b/32.png) [@Davidswm1](https://ask.bioexcel.eu/u/Davidswm1)\ **Post date:** [July 6, 2022, 3:12pm UTC](https://ask.bioexcel.eu/t/using-lipids-in-haddock/3794/1 "2022-07-06T15:12:47Z")
I have been trying to dock proteins to a protein that is associated with lipids. The docking works when the lipids are removed, but I would like to include them in case they modify the binding event. I had some success last week by defining the lipids as HETATMs (after consulting with a Japanese group that used these lipids in HADDOCK analyses before). It worked great for about a week and then yesterday, I got the following error message using the exact same .pdb files " [Errno 2] No such file or directory: ‘/tmp/tmp56sbrk5t/POV\_prodrg" I would love to get this functionality back. Was there some change in a recent update? Many thanks. ---
**Author:** ![amjjbonvin](https://dub1.discourse-cdn.com/flex013/user_avatar/ask.bioexcel.eu/amjjbonvin/32/23_2.png) [@amjjbonvin](https://ask.bioexcel.eu/u/amjjbonvin)\ **Post date:** [July 6, 2022, 4:15pm UTC](https://ask.bioexcel.eu/t/using-lipids-in-haddock/3794/2 "2022-07-06T16:15:22Z")
There was a python2.7 / python3 issue that had been introduced recently with an update. This is now solved. Please try again ---
**Author:** ![Davidswm1](https://avatars.discourse-cdn.com/v4/letter/d/3da27b/32.png) [@Davidswm1](https://ask.bioexcel.eu/u/Davidswm1)\ **Post date:** [July 6, 2022, 4:50pm UTC](https://ask.bioexcel.eu/t/using-lipids-in-haddock/3794/3 "2022-07-06T16:50:52Z")
That seems to have worked. Analysis is running now. Hopefully it is successful. Thanks for fixing the problem so quickly.