# The suitable HADDOCK server web interface for my protein

**URL:** https://ask.bioexcel.eu/t/the-suitable-haddock-server-web-interface-for-my-protein/1246
**Category:** HADDOCK
**Created:** [October 27, 2018, 7:32pm UTC](https://ask.bioexcel.eu/t/the-suitable-haddock-server-web-interface-for-my-protein/1246 "2018-10-27T19:32:39Z")
**Posts on this page:** 4
**Page:** 1

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### Author: ![sola\_sola](https://avatars.discourse-cdn.com/v4/letter/s/b5a626/32.png) [@sola\_sola](https://ask.bioexcel.eu/u/sola_sola)
#### Post date: [October 27, 2018, 7:32pm UTC](https://ask.bioexcel.eu/t/the-suitable-haddock-server-web-interface-for-my-protein/1246/1 "2018-10-27T19:32:39Z")

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Hi  
I have a subunit of homodimeric protein, and I want to build a dimer, so I used the Easy interface that gave me acceptable results. my question is this method correct or do I need to choose the Guru interface?

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### Author: ![amjjbonvin](https://dub1.discourse-cdn.com/flex013/user_avatar/ask.bioexcel.eu/amjjbonvin/32/23_2.png) [@amjjbonvin](https://ask.bioexcel.eu/u/amjjbonvin)
#### Post date: [October 29, 2018, 11:51am UTC](https://ask.bioexcel.eu/t/the-suitable-haddock-server-web-interface-for-my-protein/1246/2 "2018-10-29T11:51:58Z")

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If you are looking at a symmetrical homodimer, you might want to use the C2 + NCS symmetry option (guru interface).

Check for some hints our tutorial about about a symmetrical homotetramer docking at:

> **[HADDOCK CASP-CAPRI T70 ab-initio docking tutorial](http://www.bonvinlab.org/education/HADDOCK-CASP-CAPRI-T70/)**
>
> A small tutorial on predicting a CASP-CAPRI target using the ab-initio mode of HADDOCK

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### Author: ![sola\_sola](https://avatars.discourse-cdn.com/v4/letter/s/b5a626/32.png) [@sola\_sola](https://ask.bioexcel.eu/u/sola_sola)
#### Post date: [October 30, 2018, 7:22am UTC](https://ask.bioexcel.eu/t/the-suitable-haddock-server-web-interface-for-my-protein/1246/3 "2018-10-30T07:22:55Z")

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Thanks for useful tutorial, but I got this error  
HaddockValidationError: You must supply active and/or passive residues for your first protein.

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### Author: ![Adrimel](https://dub1.discourse-cdn.com/flex013/user_avatar/ask.bioexcel.eu/adrimel/32/40_2.png) [@Adrimel](https://ask.bioexcel.eu/u/Adrimel)
#### Post date: [October 30, 2018, 8:12am UTC](https://ask.bioexcel.eu/t/the-suitable-haddock-server-web-interface-for-my-protein/1246/4 "2018-10-30T08:12:18Z")

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Dear Sola,

What are exactly the restraints you used for your docking run? You must specify:

- `center of mass` --\> **TRUE**
- `Noncrystallographic symmetry` --\> **TRUE** and use the menu to define one pair A-B
- `C2 symmetry segment pair` --\> **TRUE** and use the menu to define one pair A-B

That should do the trick