# RESP for a cofactor

**URL:** <https://ask.bioexcel.eu/t/resp-for-a-cofactor/4212>\
**Category:** General Topics in Molecular Dynamics\
**Created:** [March 22, 2023, 12:38pm UTC](https://ask.bioexcel.eu/t/resp-for-a-cofactor/4212 "2023-03-22T12:38:04Z")\
**Posts on this page:** 1\
**Page:** 1

<div class="post-metadata">

**Author:** ![v.sheg](https://avatars.discourse-cdn.com/v4/letter/v/848f3c/32.png) [@v.sheg](https://ask.bioexcel.eu/u/v.sheg)\
**Post date:** [March 22, 2023, 12:38pm UTC](https://ask.bioexcel.eu/t/resp-for-a-cofactor/4212/1 "2023-03-22T12:38:04Z")

</div>

Hello, I want to ask you for advice.

I need to do RESP on a protein cofactor. The cofactor is strongly bound to the protein, so I am trying to decide which conformations I should use to perform charge fitting. Should I use the one conformation observed in the protein or should I generate a set of “minimal energy” conformations for a multi-fitting? What do you think?