# ProABC2 alignment fail

**URL:** <https://ask.bioexcel.eu/t/proabc2-alignment-fail/4601>\
**Category:** Uncategorized\
**Created:** [October 11, 2023, 7:36pm UTC](https://ask.bioexcel.eu/t/proabc2-alignment-fail/4601 "2023-10-11T19:36:35Z")\
**Posts on this page:** 1\
**Page:** 1

<div class="post-metadata">

**Author:** ![YeeChuen](https://avatars.discourse-cdn.com/v4/letter/y/eb8c5e/32.png) [@YeeChuen](https://ask.bioexcel.eu/u/YeeChuen)\
**Post date:** [October 11, 2023, 7:36pm UTC](https://ask.bioexcel.eu/t/proabc2-alignment-fail/4601/1 "2023-10-11T19:36:35Z")

</div>

Hi wondering what would be the error for:  
Alignment failed for chain L:  
EVVMTQSPASLSVSPGERATLSCRARASLGISTDLAWYQQRPGQAPRLLIYGASTRATGIPARFSGSGSGTEFTLTISSLQSEDSAVYYCQQYSNWPLTFGGGTKVEIK

for reference I am passing these heavy and light chain:

> Heavy Chain  
> VQLAQSGSELRKPGASVKVSCDTSGHSFTSNAIHWVRQAPGQGLEWMGWINTDTGTPTYAQGFTGRFVFSLDTSARTAYLQISSLKADDTAVFYCARERDYSDYFFDYWGQGTLVTVSS  
> Light Chain  
> EVVMTQSPASLSVSPGERATLSCRARASLGISTDLAWYQQRPGQAPRLLIYGASTRATGIPARFSGSGSGTEFTLTISSLQSEDSAVYYCQQYSNWPLTFGGGTKVEIK