# Homotetraner docking **URL:** \ **Category:** HADDOCK\ **Created:** [January 26, 2025, 3:06pm UTC](https://ask.bioexcel.eu/t/homotetraner-docking/5405 "2025-01-26T15:06:24Z")\ **Posts on this page:** 2\ **Page:** 1
**Author:** ![eisa](https://avatars.discourse-cdn.com/v4/letter/e/ba9def/32.png) [@eisa](https://ask.bioexcel.eu/u/eisa)\ **Post date:** [January 26, 2025, 3:06pm UTC](https://ask.bioexcel.eu/t/homotetraner-docking/5405/1 "2025-01-26T15:06:24Z")
Hi how i can make a new tetramer protein i have made a monomer by alfa fold now i want to make homotetraner by docking ---
**Author:** ![marco.giulini](https://dub1.discourse-cdn.com/flex013/user_avatar/ask.bioexcel.eu/marco.giulini/32/961_2.png) [@marco.giulini](https://ask.bioexcel.eu/u/marco.giulini)\ **Post date:** [January 27, 2025, 8:55am UTC](https://ask.bioexcel.eu/t/homotetraner-docking/5405/2 "2025-01-27T08:55:53Z")
hi, the procedure may depend on the information you have, but please start checking this tutorial [HADDOCK 2.4 CASP-CAPRI T70 ab-initio docking tutorial – Bonvin Lab](https://www.bonvinlab.org/education/HADDOCK24/HADDOCK24-CASP-CAPRI-T70/) which explains how to perform _ab-initio_ (i.e. with no prior information) docking of symmetrical homo-multimers. If you have some information on the interface consider adding it to the docking by means of ambiguous interaction restraints (check other tutorials such as [HADDOCK2.4 basic protein-protein docking tutorial – Bonvin Lab](https://www.bonvinlab.org/education/HADDOCK24/HADDOCK24-protein-protein-basic/)) In case you prefer to use HADDOCK3 (command line-only), have a look at this tutorial (for a symmetric homotrimer): [haddock3/examples/docking-protein-homotrimer/docking-protein-homotrimer-test.cfg at main · haddocking/haddock3 · GitHub](https://github.com/haddocking/haddock3/blob/main/examples/docking-protein-homotrimer/docking-protein-homotrimer-test.cfg) Hope it helps. Marco