# Error in uploading my DNA and protein PDB files

**URL:** <https://ask.bioexcel.eu/t/error-in-uploading-my-dna-and-protein-pdb-files/821>\
**Category:** HADDOCK\
**Created:** [March 1, 2018, 6:16am UTC](https://ask.bioexcel.eu/t/error-in-uploading-my-dna-and-protein-pdb-files/821 "2018-03-01T06:16:20Z")\
**Posts on this page:** 1\
**Showing post:** 2

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**Author:** ![amjjbonvin](https://dub1.discourse-cdn.com/flex013/user_avatar/ask.bioexcel.eu/amjjbonvin/32/23_2.png) [@amjjbonvin](https://ask.bioexcel.eu/u/amjjbonvin)\
**Post date:** [March 1, 2018, 2:21pm UTC](https://ask.bioexcel.eu/t/error-in-uploading-my-dna-and-protein-pdb-files/821/2 "2018-03-01T14:21:29Z")

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Well you must have edited your PDB file and modified the columns… So correct it. The PDB format is a strict one!

Note that the forum is searchable. Here is another thread about this problem:

> [@Haddock processing error: align pdb properly](http://ask.bioexcel.eu/t/haddock-processing-error-align-pdb-properly/750):
>
> Why do I get this error message for the protein pdb file below? First pdb file is incorrectly formatted. Please align the columns of your PDB properly. Is possible that Haddock does not allow ‘ANISOU’ lines (having to do with anisotropic temperature factors)? Note that columns were aligned before pasting into this message box). ATOM 1 N GLU A 645 36.072 64.293 35.724 1.00 54.23 N ANISOU 1 N GLU A 645 8558 6372 5675 -185 -306 -1032 N ATOM …

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_[View the full topic](https://ask.bioexcel.eu/t/error-in-uploading-my-dna-and-protein-pdb-files/821)._