# Error at neutralizing step

**URL:** <https://ask.bioexcel.eu/t/error-at-neutralizing-step/2792>\
**Category:** MDWeb\
**Created:** [January 25, 2021, 5:32am UTC](https://ask.bioexcel.eu/t/error-at-neutralizing-step/2792 "2021-01-25T05:32:43Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![Gayatri](https://avatars.discourse-cdn.com/v4/letter/g/d78d45/32.png) [@Gayatri](https://ask.bioexcel.eu/u/Gayatri)\
**Post date:** [January 25, 2021, 5:32am UTC](https://ask.bioexcel.eu/t/error-at-neutralizing-step/2792/1 "2021-01-25T05:32:43Z")

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Hi,  
I have been trying to run AMBER FULL MD SETUP, but getting stuck/ getting an error at the neutralizing step. My protein is neutral. How do I go ahead? please guide.

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**Author:** ![adam.hospital](https://dub1.discourse-cdn.com/flex013/user_avatar/ask.bioexcel.eu/adam.hospital/32/151_2.png) [@adam.hospital](https://ask.bioexcel.eu/u/adam.hospital)\
**Post date:** [January 25, 2021, 9:45pm UTC](https://ask.bioexcel.eu/t/error-at-neutralizing-step/2792/2 "2021-01-25T21:45:01Z")

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Hi Gayatri,

please check your input structure. I see in the log files disconnected residues and a chain with only one residue. All these can affect the setup.

Please contact us again if you still have problems.

Regards,

-Adam-