# Easy interface protein-peptide docking of a protein with multiple chain and getting error

**URL:** <https://ask.bioexcel.eu/t/easy-interface-protein-peptide-docking-of-a-protein-with-multiple-chain-and-getting-error/1323>\
**Category:** HADDOCK\
**Created:** [December 11, 2018, 8:04am UTC](https://ask.bioexcel.eu/t/easy-interface-protein-peptide-docking-of-a-protein-with-multiple-chain-and-getting-error/1323 "2018-12-11T08:04:03Z")\
**Posts on this page:** 3\
**Page:** 1

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**Author:** ![mitra](https://avatars.discourse-cdn.com/v4/letter/m/a3d4f5/32.png) [@mitra](https://ask.bioexcel.eu/u/mitra)\
**Post date:** [December 11, 2018, 8:04am UTC](https://ask.bioexcel.eu/t/easy-interface-protein-peptide-docking-of-a-protein-with-multiple-chain-and-getting-error/1323/1 "2018-12-11T08:04:03Z")

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Hello,  
I have a protein pdb file with 10 chain and I have changed the chain numbers continuously. ButThere was an error in rigid body stage of the docking:  
Status: FAILED  
There was an error in rigid body stage of the docking.

For more information, check the following CNS output files for error messages:  
complex\_run1\_it0\_refine\_1.out If you are unsure how to correct the error, visit the HADDOCK website, check out the HADDOCK discuss group, or send a mail to [haddock.support@gmail.com](mailto:haddock.support@gmail.com)

Thank you very much if you guide me.  
Mitra

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**Author:** ![Adrimel](https://dub1.discourse-cdn.com/flex013/user_avatar/ask.bioexcel.eu/adrimel/32/40_2.png) [@Adrimel](https://ask.bioexcel.eu/u/Adrimel)\
**Post date:** [December 12, 2018, 5:43pm UTC](https://ask.bioexcel.eu/t/easy-interface-protein-peptide-docking-of-a-protein-with-multiple-chain-and-getting-error/1323/2 "2018-12-12T17:43:33Z")

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Dear Mitra,

I am not completely sure what happened here but there is probably something wrong with your file format or the restraints format. Could you please send us the `complex_run1_it0_refine_1.out` file?

Regards,  
Adrien

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<div class="post-metadata">

**Author:** ![mitra](https://avatars.discourse-cdn.com/v4/letter/m/a3d4f5/32.png) [@mitra](https://ask.bioexcel.eu/u/mitra)\
**Post date:** [December 13, 2018, 5:39am UTC](https://ask.bioexcel.eu/t/easy-interface-protein-peptide-docking-of-a-protein-with-multiple-chain-and-getting-error/1323/3 "2018-12-13T05:39:33Z")

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Hi  
Thank you for responding.

[http://milou.science.uu.nl/serviceresults/HADDOCK2.2/4216596988/AS11/complex\_run1\_it0\_refine\_1.out](http://milou.science.uu.nl/serviceresults/HADDOCK2.2/4216596988/AS11/complex_run1_it0_refine_1.out)

I think there is a limitation on the number of chains or residues. Because I reduced the number of chains from 10 to 5 and I did not receive an error. Is it OK? if yes, so what is the maximum number of residues or chains?

I am waiting for your response

Best regards