# Domain docking failed

**URL:** <https://ask.bioexcel.eu/t/domain-docking-failed/1638>\
**Category:** HADDOCK\
**Created:** [August 14, 2019, 10:28am UTC](https://ask.bioexcel.eu/t/domain-docking-failed/1638 "2019-08-14T10:28:58Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![akukol](https://avatars.discourse-cdn.com/v4/letter/a/f04885/32.png) [@akukol](https://ask.bioexcel.eu/u/akukol)\
**Post date:** [August 14, 2019, 10:28am UTC](https://ask.bioexcel.eu/t/domain-docking-failed/1638/1 "2019-08-14T10:28:58Z")

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Hello,  
I tried to dock an inserted domain 2 to a domain 1. The domains are arranged as follows in the sequence:  
111111111111222222222222221111111111111111111111

Using the easy interface, for protein 1, I specified the two adjacent residues (213, 214) as active and for the 2nd protein I specified the N- and C-termini as active (1 and 117). Unfortunately, the docking failed with the error message below ([http://milou.science.uu.nl/serviceresults/HADDOCK2.2/5538826474/PositiveContrl](http://milou.science.uu.nl/serviceresults/HADDOCK2.2/5538826474/PositiveContrl)) :

TOTAL NUMBER OF DISTANCE RESTRAINTS FOR RIGID BODY DOCKING IS ZERO!  
CONTROL YOUR PARAMETER SETTINGS AND RESTRAINT DEFINITIONS  
STRUCTURE NUMBER 601

I would be grateful for any help, or info if that approach is possible or not.

Many thanks  
Andreas

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**Author:** ![amjjbonvin](https://dub1.discourse-cdn.com/flex013/user_avatar/ask.bioexcel.eu/amjjbonvin/32/23_2.png) [@amjjbonvin](https://ask.bioexcel.eu/u/amjjbonvin)\
**Post date:** [August 14, 2019, 11:44am UTC](https://ask.bioexcel.eu/t/domain-docking-failed/1638/2 "2019-08-14T11:44:12Z")

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Could be a mystery of the grid (on which our jobs are running)… Or bad luck in some way.

I restarted your run and it seems happily proceeding:

[http://alcazar.science.uu.nl/serviceresults/HADDOCK2.2/1111111111/test2/](http://alcazar.science.uu.nl/serviceresults/HADDOCK2.2/1111111111/test2/)
