# Docking proteins with one set of ambigious constraints **URL:** \ **Category:** HADDOCK\ **Created:** [November 2, 2022, 3:55pm UTC](https://ask.bioexcel.eu/t/docking-proteins-with-one-set-of-ambigious-constraints/3966 "2022-11-02T15:55:40Z")\ **Posts on this page:** 3\ **Page:** 1
**Author:** ![GGTheophall](https://avatars.discourse-cdn.com/v4/letter/g/b782af/32.png) [@GGTheophall](https://ask.bioexcel.eu/u/GGTheophall)\ **Post date:** [November 2, 2022, 3:55pm UTC](https://ask.bioexcel.eu/t/docking-proteins-with-one-set-of-ambigious-constraints/3966/1 "2022-11-02T15:55:40Z")
The HADDOCK category is meant to discuss any HADDOCK-related issue. For general information about HADDOCK refer to [HADDOCK – Bonvin Lab](http://www.bonvinlab.org/software/haddock2.4) I am trying to dock two proteins, A and B. I have NMR data supplying ambiguous constraints for protein A, but no ambiguous constraints for protein B. I have MS data that gives me unambiguous constrains for both proteins A and B. HADDOCK will not let me submit a calculation with only protein A having ambiguous constraints. ---
**Author:** ![amjjbonvin](https://dub1.discourse-cdn.com/flex013/user_avatar/ask.bioexcel.eu/amjjbonvin/32/23_2.png) [@amjjbonvin](https://ask.bioexcel.eu/u/amjjbonvin)\ **Post date:** [November 2, 2022, 4:10pm UTC](https://ask.bioexcel.eu/t/docking-proteins-with-one-set-of-ambigious-constraints/3966/2 "2022-11-02T16:10:09Z")
Define A as active and the entire surface of B as passive (the server has an auutomatic option for this) And add your MS data as unambiguous restraints Does required expert level access. ---
**Author:** ![GGTheophall](https://avatars.discourse-cdn.com/v4/letter/g/b782af/32.png) [@GGTheophall](https://ask.bioexcel.eu/u/GGTheophall)\ **Post date:** [November 2, 2022, 8:28pm UTC](https://ask.bioexcel.eu/t/docking-proteins-with-one-set-of-ambigious-constraints/3966/3 "2022-11-02T20:28:39Z")
Worked like a charm! Thank you so much for the speedy response and free program!!!