# Cannot repeat the HADDCOK example (E2A-HPR)

**URL:** <https://ask.bioexcel.eu/t/cannot-repeat-the-haddcok-example-e2a-hpr/1966>\
**Category:** HADDOCK\
**Created:** [April 28, 2020, 4:00am UTC](https://ask.bioexcel.eu/t/cannot-repeat-the-haddcok-example-e2a-hpr/1966 "2020-04-28T04:00:02Z")\
**Posts on this page:** 6\
**Page:** 1

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**Author:** ![zuowanwang](https://avatars.discourse-cdn.com/v4/letter/z/ecc23a/32.png) [@zuowanwang](https://ask.bioexcel.eu/u/zuowanwang)\
**Post date:** [April 28, 2020, 4:00am UTC](https://ask.bioexcel.eu/t/cannot-repeat-the-haddcok-example-e2a-hpr/1966/1 "2020-04-28T04:00:02Z")

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Hello all,

I try to use the HADDOCK web server. First I tested the E2A-HPR example, followed the steps, and downloaded the results. When I compared the result and identified crystal complex (1GGR), the top-rated pose cannot overlap well with 1GGR. So I downloaded the example result file from the website and did the comparison again, this time the two can overlap very well (see the attached pics). I’m wondering whether it’s a normal situation or I made some mistakes. Thank you!

 ![Picture1](https://europe1.discourse-cdn.com/flex013/uploads/bioexcel/original/1X/5da34a5821b8bb40f63eecb09324d7e8d5e0c7f6.jpeg)

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**Author:** ![amjjbonvin](https://dub1.discourse-cdn.com/flex013/user_avatar/ask.bioexcel.eu/amjjbonvin/32/23_2.png) [@amjjbonvin](https://ask.bioexcel.eu/u/amjjbonvin)\
**Post date:** [April 28, 2020, 6:49am UTC](https://ask.bioexcel.eu/t/cannot-repeat-the-haddcok-example-e2a-hpr/1966/2 "2020-04-28T06:49:59Z")

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Did you following the tutorial instructions step by step?

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<div class="post-metadata">

**Author:** ![zuowanwang](https://avatars.discourse-cdn.com/v4/letter/z/ecc23a/32.png) [@zuowanwang](https://ask.bioexcel.eu/u/zuowanwang)\
**Post date:** [April 28, 2020, 7:05am UTC](https://ask.bioexcel.eu/t/cannot-repeat-the-haddcok-example-e2a-hpr/1966/3 "2020-04-28T07:05:58Z")

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Yes, I followed step by step and the protein is prepared via PyMol. Thanks

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**Author:** ![amjjbonvin](https://dub1.discourse-cdn.com/flex013/user_avatar/ask.bioexcel.eu/amjjbonvin/32/23_2.png) [@amjjbonvin](https://ask.bioexcel.eu/u/amjjbonvin)\
**Post date:** [April 28, 2020, 7:20am UTC](https://ask.bioexcel.eu/t/cannot-repeat-the-haddcok-example-e2a-hpr/1966/4 "2020-04-28T07:20:44Z")

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Well - what can I say… Did you check other clusters?

Or try again

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<div class="post-metadata">

**Author:** ![zuowanwang](https://avatars.discourse-cdn.com/v4/letter/z/ecc23a/32.png) [@zuowanwang](https://ask.bioexcel.eu/u/zuowanwang)\
**Post date:** [April 28, 2020, 7:35am UTC](https://ask.bioexcel.eu/t/cannot-repeat-the-haddcok-example-e2a-hpr/1966/5 "2020-04-28T07:35:49Z")

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I’ve checked top five clusters, not found overlap one. I’d like to try again and will update my result. Thanks

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<div class="post-metadata">

**Author:** ![zuowanwang](https://avatars.discourse-cdn.com/v4/letter/z/ecc23a/32.png) [@zuowanwang](https://ask.bioexcel.eu/u/zuowanwang)\
**Post date:** [April 29, 2020, 2:28am UTC](https://ask.bioexcel.eu/t/cannot-repeat-the-haddcok-example-e2a-hpr/1966/6 "2020-04-29T02:28:58Z")

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Hello, I’ve checked other clusters first, and got overlap clusters (7 and 8), the 1-5 clusters have no good ovelap with 1GGR. Meanwhile, I submited another run and waiting for the result to see whether the top-rated cluster can overlap with 1GGR well. Thanks