# Biotechnology1370

**URL:** <https://ask.bioexcel.eu/t/biotechnology1370/533>\
**Category:** HADDOCK\
**Created:** [October 12, 2017, 4:37pm UTC](https://ask.bioexcel.eu/t/biotechnology1370/533 "2017-10-12T16:37:09Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![mohammad](https://avatars.discourse-cdn.com/v4/letter/m/e274bd/32.png) [@mohammad](https://ask.bioexcel.eu/u/mohammad)\
**Post date:** [October 12, 2017, 4:37pm UTC](https://ask.bioexcel.eu/t/biotechnology1370/533/1 "2017-10-12T16:37:09Z")

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**why when work with haddock its error show?**

**Your** prediction FAILED

Error message: PDB file contains multiple chains with overlapping numbering: A237 - B237  
If this message is unclear, please contact a.m.j.j.bonvin@uu.nl

Full error message:_emphasized text_  
Traceback (most recent call last):  
File “./cportdaemon.py”, line 48, in  
pdb = conversion.validate\_pdb(pdbs[0], form.pdb.chain)  
File “/home/enmr/services/CPORT/cron/conversion.py”, line 52, in validate\_pdb  
raise HaddockValidationError("%s contains multiple chains with overlapping numbering: %s%s - %s%s" % (dataname,firstchain, resnr.strip(), currchain, resnr.strip()))  
ValueError: PDB file contains multiple chains with overlapping numbering: A237 - B237

PDB file contains multiple chains with overlapping numbering: A237 - B237

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<div class="post-metadata">

**Author:** ![mtrellet](https://dub1.discourse-cdn.com/flex013/user_avatar/ask.bioexcel.eu/mtrellet/32/42_2.png) [@mtrellet](https://ask.bioexcel.eu/u/mtrellet)\
**Post date:** [October 20, 2017, 1:15pm UTC](https://ask.bioexcel.eu/t/biotechnology1370/533/2 "2017-10-20T13:15:49Z")

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Hello Mohammad,

As it is reported, you need to have a unique numbering within a single molecule, even with multiple chains. So you might want to renumber your second chain, starting from the last residue ID of the 1st chain + 1.