# SPOTON

**URL:** https://ask.bioexcel.eu/c/spoton/18.md

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## [About the SPOTON category](https://ask.bioexcel.eu/t/about-the-spoton-category/1130)

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**Author:** [@mtrellet](https://ask.bioexcel.eu/u/mtrellet)\
**Replies:** 0

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This is the forum for the SpotOn web portalwhich implements a robust algorithm developed to identify and classify the interfacial residues as Hot-Spots (HS) and Null-Spots (NS).

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## [SpotOn Webserver Version Number?](https://ask.bioexcel.eu/t/spoton-webserver-version-number/5857)

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**Author:** [@Kristian](https://ask.bioexcel.eu/u/Kristian)\
**Replies:** 1\
**Last updated:** [August 9, 2025, 5:20pm UTC](https://ask.bioexcel.eu/t/spoton-webserver-version-number/5857 "2025-08-09T17:20:51Z")

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To the team, I’m required to mention the version number of SpotOn used in my research paper preceding publication. I used the SpotOn Webserver on the 26th September 2023 - if there is a specific version number for SpotOn…

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## [Spoton error message](https://ask.bioexcel.eu/t/spoton-error-message/5255)

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**Author:** [@jin](https://ask.bioexcel.eu/u/jin)\
**Replies:** 4\
**Last updated:** [September 19, 2024, 8:51am UTC](https://ask.bioexcel.eu/t/spoton-error-message/5255 "2024-09-19T08:51:51Z")

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I submitted a protein-peptide pdb file with chain A and C. and got this error message to contact administrator. This is my first time to use Spoton. I am wondering if someone could please help me with this. the job id i…

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## [SpotON/EGI-CheckIn not working](https://ask.bioexcel.eu/t/spoton-egi-checkin-not-working/4553)

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**Author:** [@Kristian](https://ask.bioexcel.eu/u/Kristian)\
**Replies:** 4\
**Last updated:** [September 26, 2023, 5:31pm UTC](https://ask.bioexcel.eu/t/spoton-egi-checkin-not-working/4553 "2023-09-26T17:31:47Z")

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To the team, I’ve resgistered for a Check-In account (which appears to be successful as per my first image attachment). But, I can’t verify this on the spotOn job submission page (refer to second image). When I manually…

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## [Different hotspots are predicted by SpotOn when the chain IDs are reversed](https://ask.bioexcel.eu/t/different-hotspots-are-predicted-by-spoton-when-the-chain-ids-are-reversed/3807)

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**Author:** [@AdiPat48](https://ask.bioexcel.eu/u/AdiPat48)\
**Replies:** 4\
**Last updated:** [July 23, 2022, 6:56pm UTC](https://ask.bioexcel.eu/t/different-hotspots-are-predicted-by-spoton-when-the-chain-ids-are-reversed/3807 "2022-07-23T18:56:46Z")

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I have noticed that when I switch the chains A and B in the submission form, the predicted results change. For instance, please consider this example - I submitted a pdb file for PDB structure 1A22 When I submitted Cha…

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## [Error submitting SpotOn Job](https://ask.bioexcel.eu/t/error-submitting-spoton-job/2774)

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**Author:** [@Kris](https://ask.bioexcel.eu/u/Kris)\
**Replies:** 5\
**Last updated:** [May 22, 2021, 7:06pm UTC](https://ask.bioexcel.eu/t/error-submitting-spoton-job/2774 "2021-05-22T19:06:00Z")

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Hi all, This is my first time using the server so it could be an issue on my side - when I try to submit a job for SpotOn i’m getting the error message: ‘Internal Server Error’ ‘The server encountered an internal erro…

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## [Spot On error message](https://ask.bioexcel.eu/t/spot-on-error-message/2180)

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**Author:** [@fidelormz](https://ask.bioexcel.eu/u/fidelormz)\
**Replies:** 1\
**Last updated:** [June 19, 2020, 8:46am UTC](https://ask.bioexcel.eu/t/spot-on-error-message/2180 "2020-06-19T08:46:59Z")

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Hello everyone! I’ve been trying to submit some inputs in SpotOn for several times during the last few weeks but I got the following error message: “The server encountered an internal error and was unable to complete yo…
