For a project I'm doing I want to make a complex of LDLR and ApoE4. In order to get ApoE4 I mutated ApoE3 because there is no Apo4 structure. The only change that had to be made was to mutate Cys112 to Arg112.
I made a complex of ApoE3 and LDLR. And I wanted to make a complex of ApoE4 and LDLR as well. However when I try to do that I get the following error:
First pdb file contains multiple residues with number 299 in chain A
Even though there is only one residue with number 299 (which is also my last residue). I tried using only 1 NMR structure instead of all 20 but it still gives the same error. Except for the mutation on residue 112 there are no differences between ApoE3 and ApoE4 so I don't know why it works for ApoE3 but not for ApoE4.
How could I solve this problem?